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Focused Ultrasound Modifications of Neural Circuit Activity in a Mammalian Brain

      Abstract

      The application of focused, pulsed ultrasound was studied as a method of modifying the activity of a local neural circuit of the mammalian brain. An in vitro hippocampal preparation was used to facilitate delivery, dosimetry and assessment of mechanisms of ultrasound effects. Extracellular evoked potentials were recorded from cell and dendritic layers of the rat hippocampal dentate gyrus. Focused pulses of ultrasound with center frequency of 500 kHz and repetition rate of 200 kHz were studied and found both to enhance and to depress electrically evoked field potentials. The fiber volley and cell population potentials were depressed, whereas the dendritic potential was enhanced. Results suggest a simultaneous mechanical and thermal mechanism of ultrasound in modifying evoked field potentials of dentate local circuits.

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      References

        • Adrianov O.S.
        • Vykhodtseva N.I.
        • Fokin V.F.
        • Avirom V.M.
        Method of local application of focused ultrasound to deep brain structures of an unrestrained unanesthetized animal.
        Byulleten Eksperimental noi Biologii i Meditsiny. 1984; 98: 115-117
        • Adrianov O.S.
        • Vykhodtseva N.I.
        • Fokin V.F.
        • et al.
        Reversible functional blocking of the optic tract by focused ultrasound.
        Byulleten Eksperimental noi Biologii i Meditsiny. 1984; 97: 760-762
      1. ANSI. The American National Standard Method for the Calibration of Microphones (ANSI Pub. #S1.10-1966, R1976). New York: American National Standards Institute, 1976.

        • Carstensen E.G.
        Self-reciprocity calibration of electroacoustic transducers.
        J Acoust Soc Am. 1947; 19: 702-710
        • Dingledine R.
        • Somjen G.
        Calcium dependance of synaptic transmission in the hippocampal slice.
        Brain Res. 1981; 207: 218-222
        • Gavrilov L.R.
        • Gersuni G.V.
        • Ilyinski O.B.
        • Tsirulnikov E.M.
        • Shchekanov E.E.
        A study of reception with the use of focused ultrasound. II. Effects on the animal receptor structures.
        Brain Res. 1977; 135: 279-285
        • Gavrilov L.R.
        • Gersuni G.V.
        • Ilyinski O.B.
        • Tsirulnikov E.M.
        • Shchekanov E.E.
        A study of reception with the use of focused ultrasound. I. Effects on the skin and deep receptor structures in man.
        Brain Res. 1977; 135: 265-277
        • Gavrilov L.R.
        Use of focused ultrasound for stimulation of nerve structures.
        Ultrasonics. 1984; 22: 132-138
      2. Jones JP. On the scattering of sound by sound, PhD thesis. Providence, RI: Brown University, 1970.

        • Mihran R.T.
        • Barnes F.S.
        • Wachtel H.
        Temporally-specific modification of myelinated axon excitability in vitro following a single ultrasound pulse.
        Ultrasound Med Biol. 1990; 16: 297-309
        • Moser E.
        • Mathiesen I.
        • Andersen P.
        Association between brain temperature and dentate field potentials in exploring and swimming rats.
        Science. 1993; 259: 1324-1326
        • Rinaldi P.C.
        • Jones J.P.
        • Reines F.
        • Price L.R.
        Modification by focused ultrasound pulses of electrically evoked responses from an in vitro hippocampal preparation.
        Brain Res. 1991; 558: 36-42
        • Schiff S.J.
        • Somjen G.G.
        The effects of temperature on synaptic transmission in hippocampal tissue slices.
        Brain Res. 1985; 345: 279-284
        • Vartanyan N.A.
        • Gavrilov L.R.
        • Zharzkaya V.D.
        • Ratnikova G.I.
        • Tsirul’nikov E.M.
        The stimulating effect of focused ultrasound on the auditory nerve fiber of the frog rana temporaria.
        Zhurnal Evolyutsionnoi Biokhimii i Fiziologii. 1981; 17: 512-518