Objective
Ultrasound (US)–targeted microbubble (MB) cavitation (UTMC)–mediated therapies have
been found to restore perfusion and enhance drug/gene delivery. Because of the potentially
longer circulation time and relative ease of storage and reconstitution of polymer-shelled
MBs compared with lipid MBs, we investigated the dynamic behavior of polymer microbubbles
and their therapeutic potential for sonoreperfusion (SRP) therapy.
Methods
The fate of polymer MBs during a single long tone-burst exposure (1 MHz, 5 ms) at
various acoustic pressures and MB concentrations was recorded via high-speed microscopy
and passive cavitation detection (PCD). SRP efficacy of the polymer MBs was investigated
in an in vitro flow system and compared with that of lipid MBs.
Discussion
Microscopy videos indicated that polymer MBs formed gas-filled clusters that continued
to oscillate, fragment and form new gas-filled clusters during the single US burst.
PCD confirmed continued acoustic activity throughout the 5-ms US excitation. SRP efficacy
with polymer MBs increased with pulse duration and acoustic pressure similarly to
that with lipid MBs but no significant differences were found between polymer and
lipid MBs.
Conclusion
These data suggest that persistent cavitation activity from polymer MBs during long
tone-burst US excitation confers excellent reperfusion efficacy.
Keywords
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Article info
Publication history
Published online: January 24, 2023
Accepted:
December 20,
2022
Received in revised form:
December 19,
2022
Received:
July 14,
2022
Publication stage
In Press Corrected ProofIdentification
Copyright
© 2022 World Federation for Ultrasound in Medicine & Biology. All rights reserved.
