Advertisement

Evaluating Atherosclerosis of the Abdominal Aorta in Rabbits Using 2-D Strain Imaging

  • Zhizhi Dong
    Affiliations
    Department of Ultrasound, First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, Yichang, China

    Central Laboratory, First College of Clinical Medical Science, China Three Gorges University & Yichang Central People's Hospital, Yichang, China
    Search for articles by this author
  • Yun Zhao
    Affiliations
    Medical College of China Three Gorges University, Yichang, China
    Search for articles by this author
  • Yue Chen
    Affiliations
    Department of Ultrasound, First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, Yichang, China

    Central Laboratory, First College of Clinical Medical Science, China Three Gorges University & Yichang Central People's Hospital, Yichang, China
    Search for articles by this author
  • Zulin Liu
    Affiliations
    Department of Ultrasound, First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, Yichang, China
    Search for articles by this author
  • Haiying Song
    Affiliations
    Department of Ultrasound, First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, Yichang, China
    Search for articles by this author
  • Hao Li
    Affiliations
    Department of Gastrointestinal Surgery, First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, Yichang, China
    Search for articles by this author
  • Douzi Shi
    Affiliations
    Department of Ultrasound, First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, Yichang, China

    Central Laboratory, First College of Clinical Medical Science, China Three Gorges University & Yichang Central People's Hospital, Yichang, China
    Search for articles by this author
  • Chang Zhou
    Affiliations
    Department of Ultrasound, First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, Yichang, China
    Search for articles by this author
  • Jun Zhou
    Affiliations
    Department of Ultrasound, First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, Yichang, China
    Search for articles by this author
  • Rong Liu
    Correspondence
    Address correspondence to: Rong Liu, Department of Ultrasound, First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, No. 183, Yiling Avenue, Yichang City, Hubei Province, Yichang, Hubei 443003, China.
    Affiliations
    Department of Ultrasound, First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People's Hospital, Yichang, China
    Search for articles by this author

      Abstract

      After establishment of an animal model of atherosclerosis, speckle tracking imaging was performed to analyze the correlation between ultrasound characteristics and pathological manifestations. Rabbits were divided into the normal control (NC) and atherosclerosis (AS) groups. Rabbits in the AS group were subjected to ultrasound-guided balloon injury of the abdominal aorta and fed a high-fat diet for 16 wk. Rabbits in the NC group were fed a normal diet for the same period. After 16 wk, all animals underwent serological tests, ultrasound and speckle tracking circumferential strain analysis. In the AS group, 28 hypo-echoic plaques had formed. The circumferential strain of six segments at the short axis of plaques in the AS group was lower than that in the NC group (p < 0.001), and global circumferential strain (GCS) in the AS group was significantly reduced compared with the NC group (p < 0.001). In the AS group, the area ratio of type I to type III collagen fibers was smaller than that in the NC group. The GCS of atherosclerotic plaques was positively correlated with the area ratio of type I to type III collagen fibers in plaques (r = 0.7181, p < 0.001). In conclusion, there is a significant positive correlation between the decreased circumferential strain and the decreased area ratio of type I to type III collagen fibers in hypo-echoic plaques.

      Key Words

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Ultrasound in Medicine and Biology
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Aboonabi A
        • Meyer RR
        • Singh I.
        The association between metabolic syndrome components and the development of atherosclerosis.
        J Hum Hypertens. 2019; 33: 844-855
        • Akcılar R
        • Emel Koçak F
        • Şimşek H
        • Akcılar A
        • Bayat Z
        • Ece E
        • Kökdaşgil H
        The effect of adropin on lipid and glucose metabolism in rats with hyperlipidemia.
        Iran J Basic Med Sci. 2016; 19: 245-251
        • Ariza de Schellenberger A
        • Poller WC
        • Stangl V
        • Landmesser U
        • Schellenberger E.
        Macrophage uptake switches on OCT contrast of superparamagnetic nanoparticles for imaging of atherosclerotic plaques.
        Int J Nanomed. 2018; 13: 7905-7913
        • Baumgartner C
        • Brandl J
        • Münch G
        • Ungerer M.
        Rabbit models to study atherosclerosis and its complications—Transgenic vascular protein expression in vivo.
        Prog Biophys Mol Biol. 2016; 121: 131-141
        • Bayturan O
        • Tuzcu EM
        • Nicholls SJ
        • Balog C
        • Lavoie A
        • Uno K
        • Crowe TD
        • Magyar WA
        • Wolski K
        • Kapadia S
        • Nissen SE
        • Schoenhagen P.
        Attenuated plaque at nonculprit lesions in patients enrolled in intravascular ultrasound atherosclerosis progression trials.
        JACC Cardiovasc Interv. 2009; 2: 672-678
        • Chrencik MT
        • Khan AA
        • Luther L
        • Anthony L
        • Yokemick J
        • Patel J
        • Sorkin JD
        • Sikdar S
        • Lal BK.
        Quantitative assessment of carotid plaque morphology (geometry and tissue composition) using computed tomography angiography.
        J Vasc Surg. 2019; 70: 858-868
        • Chowdhury MM
        • Tarkin JM
        • Evans NR
        • Le E
        • Warburton EA
        • Hayes PD
        • Rudd J
        • Coughlin PA.
        18F-FDG uptake on PET/CT in symptomatic versus asymptomatic carotid disease: A meta-analysis.
        Eur J Vasc Endovasc Surg. 2018; 56: 172-179
        • Davrieux CF
        • Giménez ME
        • González CA
        • Ancel A
        • Guinin M
        • Fahrer B
        • Serra E
        • Kwak JM
        • Marescaux J
        • Hostettler A.
        Mixed reality navigation system for ultrasound-guided percutaneous punctures: A pre-clinical evaluation.
        Surg Endosc. 2020; 34: 226-230
        • Feng L
        • Hua C
        • Sun H
        • Qin LY
        • Niu PP
        • Guo ZN
        • Yang Y.
        Association between serum uric acid level and carotid atherosclerosis in Chinese individuals aged 75 years or older: A hospital-based case–control study.
        J Nutr Health Aging. 2018; 22: 508-512
        • Gajdova J
        • Karasek D
        • Goldmannova D
        • Krystynik O
        • Schovanek J
        • Vaverkova H
        • Zadrazil J.
        Pulse wave analysis and diabetes mellitus: A systematic review.
        Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017; 161: 223-233
        • Gao Y
        • Peng J
        • Ren Z
        • He NY
        • Li Q
        • Zhao XS
        • Wang MM
        • Wen HY
        • Tang ZH
        • Jiang ZS
        • Wang GX
        • Liu LS.
        Functional regulatory roles of microRNAs in atherosclerosis.
        Clin Chim Acta. 2016; 460: 164-171
        • Holm Nielsen S
        • Jonasson L
        • Kalogeropoulos K
        • Karsdal MA
        • Reese-Petersen AL
        • Auf dem Keller U
        • Genovese F
        • Nilsson J
        • Goncalves I
        Exploring the role of extracellular matrix proteins to develop biomarkers of plaque vulnerability and outcome.
        J Intern Med. 2020; 287: 493-513
        • Hopkins PN.
        Molecular biology of atherosclerosis.
        Physiol Rev. 2013; 93: 1317-1542
        • Johri AM
        • Herr JE
        • Li TY
        • Yau O
        • Nambi V.
        Novel ultrasound methods to investigate carotid artery plaque vulnerability.
        J Am Soc Echocardiogr. 2017; 30: 139-148
        • Kimura Y
        • Yanagida T
        • Onda A
        • Tsukui D
        • Hosoyamada M
        • Kono H.
        Soluble uric acid promotes atherosclerosis via AMPK (AMP-activated protein kinase)-mediated inflammation.
        Arterioscler Thromb Vasc Biol. 2020; 40: 570-582
        • Kobiyama K
        • Ley K.
        Atherosclerosis.
        Circ Res. 2018; 123: 1118-1120
        • Lamacchia O
        • Sorrentino MR.
        Diabetes mellitus, arterial stiffness and cardiovascular disease: Clinical implications and the influence of SGLT2i.
        Curr Vasc Pharmacol. 2020; 18: 1-8
        • Larsson M
        • Heyde B
        • Kremer F
        • Brodin LÅ
        • D'hooge J
        Ultrasound speckle tracking for radial, longitudinal and circumferential strain estimation of the carotid artery—An in vitro validation via sonomicrometry using clinical and high-frequency ultrasound.
        Ultrasonics. 2015; 56: 399-408
        • Libby P
        • Buring JE
        • Badimon L
        • Hansson GK
        • Deanfield J
        • Bittencourt MS
        • Tokgözoğlu L
        • Lewis EF.
        Atherosclerosis.
        Nat Rev Dis Primers. 2019; 5: 56
        • Li F
        • McDermott MM
        • Li D
        • Carroll TJ
        • Hippe DS
        • Kramer CM
        • Fan Z
        • Zhao X
        • Hatsukami TS
        • Chu B
        • Wang J
        • Yuan C.
        The association of lesion eccentricity with plaque morphology and components in the superficial femoral artery: A high-spatial-resolution, multi-contrast weighted CMR study.
        J Cardiovasc Magn Reson. 2010; 12: 37
        • Motoyama R
        • Saito K
        • Tonomura S
        • Ishibashi-Ueda H
        • Yamagami H
        • Kataoka H
        • Morita Y
        • Uchihara Y
        • Iihara K
        • Takahashi JC
        • Sugie K
        • Toyoda K
        • Nagatsuka K.
        Utility of complementary magnetic resonance plaque imaging and contrast-enhanced ultrasound to detect carotid vulnerable plaques.
        J Am Heart Assoc. 2019; 8e011302
        • Nguyen DM
        • Wagenhäuser MU
        • Mehrkens D
        • Adam M
        • Tsao PS
        • Ramasubramanian AK.
        An automated algorithm to quantify collagen distribution in aortic wall.
        J Histochem Cytochem. 2019; 67: 267-274
        • Patton DM
        • Li T
        • Hétu MF
        • Day AG
        • Preece E
        • Matangi MF
        • Johri AM.
        Speckle tracking carotid artery circumferential strain is a marker of arterial sclerosis but not coronary atherosis.
        J Clin Ultrasound. 2018; 46: 575-581
        • Rafailidis V
        • Chryssogonidis I
        • Xerras C
        • Grisan E
        • Cheimariotis GA
        • Tegos T
        • Rafailidis D
        • Sidhu PS
        • Charitanti-Kouridou A.
        An ultrasonographic multiparametric carotid plaque risk index associated with cerebrovascular symptomatology: A study comparing color Doppler imaging and contrast-enhanced ultrasonography.
        AJNR Am J Neuroradiol. 2019; 40: 1022-1028
        • Rafailidis V
        • Chryssogonidis I
        • Xerras C
        • Nikolaou I
        • Tegos T
        • Kouskouras K
        • Rafailidis D
        • Charitanti-Kouridou A.
        A comparative study of color Doppler imaging and contrast-enhanced ultrasound for the detection of ulceration in patients with carotid atherosclerotic disease.
        Eur Radiol. 2019; 29: 2137-2145
        • Saba L
        • Saam T
        • Jäger HR
        • Yuan C
        • Hatsukami TS
        • Saloner D
        • Wasserman BA
        • Bonati LH
        • Wintermark M.
        Imaging biomarkers of vulnerable carotid plaques for stroke risk prediction and their potential clinical implications.
        Lancet Neurol. 2019; 18: 559-572
        • Shami A
        • Gonçalves I
        Hultgårdh-Nilsson A. Collagen and related extracellular matrix proteins in atherosclerotic plaque development.
        Curr Opin Lipidol. 2014; 25: 394-399
        • Wilson RB
        • Miller RA
        • Middleton CC
        • Kinden D.
        Atherosclerosis in rabbits fed a low cholesterol diet for five years.
        Arteriosclerosis. 1982; 2: 228-241
        • Witthoft A
        • Yazdani A
        • Peng Z
        • Bellini C
        • Humphrey JD
        • Karniadakis GE.
        A discrete mesoscopic particle model of the mechanics of a multi-constituent arterial wall.
        J R Soc Interface. 2016; 1320150964
        • Yuan Y
        • Huang F
        • Lin F
        • Lin M
        • Zhu P.
        Association of high serum uric acid and increased arterial stiffness is dependent on cardiovascular risk factors in female population.
        Horm Metab Res. 2019; 51: 367-374
        • Zamani M
        • Skagen K
        • Scott H
        • Russell D
        • Skjelland M.
        Advanced ultrasound methods in assessment of carotid plaque instability: A prospective multimodal study.
        BMC Neurol. 2020; 20: 39
        • Zhang ZK
        • Li J
        • Yan DX
        • Leung WN
        • Zhang BT.
        Icaritin inhibits collagen degradation-related factors and facilitates collagen accumulation in atherosclerotic lesions: A potential action for plaque stabilization.
        Int J Mol Sci. 2016; 17: 169
        • Zhang T
        • Su G
        • Mi SH
        • Yang HX
        • Xin W
        • Dai WL
        • Liu JH.
        Association between blood glucose variability and the. characteristics of vulnerable plaque in elderly non-ST segment elevation acute coronary syndrome patients.
        Int Heart J. 2019; 60: 569-576
        • Zhao T
        • Gong HP
        • Dong ZQ
        • Du YM
        • Lu QH
        • Chen HQ.
        Predictive value of fasting blood glucose for serious coronary atherosclerosis in non-diabetic patients.
        J Int Med Res. 2019; 47: 152-158