Abstract
The aim of this study was to determine whether pre-treatment with nitric oxide-loaded
echogenic liposomes (NO-ELIP) plus ultrasound can improve highlighting by molecularly
targeted (anti-vascular cell adhesion molecule 1 [VCAM-1]) ELIP of atheroma components.
Atherosclerotic animals were treated with anti-VCAM-1-ELIP or immunoglobulin (IgG)-ELIP.
Each group was selected at random to receive pre-treatment with standard ELIP plus
ultrasound, NO-ELIP without ultrasound and NO-ELIP plus ultrasound. Intravascular
ultrasound highlighting data for the same arterial segments were collected before
and after treatment. Pre-treatment with NO-ELIP plus ultrasound resulted in a significant
increase in acoustic enhancement by anti-VCAM-1-ELIP (21.3 ± 1.5% for gray-scale value,
53.9 ± 3.1% for radiofrequency data; p < 0.001 vs. IgG-ELIP, p < 0.05 vs. pre-treatment with standard ELIP plus ultrasound or NO-ELIP without ultrasound).
NO-ELIP plus ultrasound can improve highlighting of atheroma by anti-VCAM-1 ELIP.
This NO pre-treatment strategy may be useful in optimizing contrast agent delivery
to the vascular wall for both diagnostic and therapeutic applications.
Key Words
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References
- Nitric oxide mediates lung vascular permeability and lymph-borne IL-6 after an intestinal ischemic insult.Shock. 2009; 32: 55-61
- Molecular imaging of vascular cell adhesion molecule-1 expression in experimental atherosclerotic plaques with radiolabelled B2702-p.Eur J Nucl Med Mol Imaging. 2007; 34: 830-840
- A major role for VCAM-1, but not ICAM-1, in early atherosclerosis.J Clin Invest. 2001; 107: 1255-1262
- Adhesion of monocytes to arterial endothelium and initiation of atherosclerosis are critically dependent on vascular cell adhesion molecule-1 gene dosage.Arterioscler Thromb Vasc Biol. 2001; 21: 1662-1667
- In vivo targeting of acoustically reflective liposomes for intravascular and transvascular ultrasonic enhancement.J Am Coll Cardiol. 1999; 33: 867-875
- The NO cascade, eNOS location, and micro-vascular permeability.Cardiovasc Res. 2010; 87: 254-261
- Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries.J Vasc Surg. 2004; 40: 115-122
- Vascular adhesion molecules in atherosclerosis.Arterioscler Thromb Vasc Biol. 2007; 27: 2292-2301
- Intravascular ultrasound molecular imaging of atheroma components in vivo.J Am Coll Cardiol. 2004; 43: 453-460
- Liposomes as ultrasound imaging contrast agents and as ultrasound-sensitive drug delivery agents.Cell Mol Biol Lett. 2002; 7: 233-235
- Nitric oxide-loaded echogenic liposomes for nitric oxide delivery and inhibition of intimal hyperplasia.J Am Coll Cardiol. 2009; 54: 652-659
- A method to co-encapsulate gas and drugs in liposomes for ultrasound-controlled drug delivery.Ultrasound Med Biol. 2008; 34: 1272-1280
- Adhesion molecules and atherogenesis.Acta Physiol Scand. 2001; 173: 35-43
- Patterns of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 expression in rabbit and mouse atherosclerotic lesions and at sites predisposed to lesion formation.Circ Res. 1999; 85: 199-207
- Expression of the adhesion molecules ICAM, VCAM, and ELAM in the arteriosclerotic plaque.Gen Diagn Pathol. 1996; 141: 289-294
- Molecular imaging of the initial inflammatory response in atherosclerosis: Implications for early detection of disease.Arterioscler Thromb Vasc Biol. 2010; 30: 54-59
- Molecular imaging of inflammation in atherosclerosis with targeted ultrasound detection of vascular cell adhesion molecule-1.Circulation. 2007; 116: 276-284
- Nitric oxide pre-treatment enhances atheroma component highlighting in vivo with ICAM-1 targeted echogenic liposomes.Ultrasound Med Biol. 2014; 40: 1167-1176
- Detection of vascular adhesion molecule-1 expression using a novel multimodal nanoparticle.Circ Res. 2005; 96: 327-336
- Nitric oxide improves molecular imaging of inflammatory atheroma using targeted echogenic immunoliposomes.Atherosclerosis. 2013; 231: 252-260
- In vivo volumetric intravascular ultrasound visualization of early/inflammatory arterial atheroma using targeted echogenic immunoliposomes.Invest Radiol. 2010; 45: 685-691
- Quantitative immunoblot assay for assessment of liposomal antibody conjugation efficiency.Anal Biochem. 2002; 300: 46-52
- Liposomal modular complexes for simultaneous targeted delivery of bioactive gases and therapeutics.J Control Release. 2010; 142: 326-331
- Acoustic characterization of echogenic liposomes: frequency-dependent attenuation and backscatter.J Acoust Soc Am. 2011; 130: 3472-3481
- Thrombolytic efficacy of tissue plasminogen activator-loaded echogenic liposomes in a rabbit thrombus model.Thromb Res. 2012; 130: 629-635
- Preparation of liposomes.in: Torchilin V.P. Weissig V. Liposomes. Oxford University Press, New York2003
- Magnetic resonance imaging of endothelial adhesion molecules in mouse atherosclerosis using dual-targeted microparticles of iron oxide.Arterioscler Thromb Vasc Biol. 2008; 28: 77-83
- Expression of PDGF-A, TGFb and VCAM-1 during the developmental stages of experimental atherosclerosis.Eur Surg Res. 2001; 33: 264-269
- Noninvasive vascular cell adhesion molecule-1 imaging identifies inflammatory activation of cells in atherosclerosis.Circulation. 2006; 114: 1504-1511
- Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the ApoE-deficient mouse.Arterioscler Thromb Vasc Biol. 1998; 18: 842-851
- Vascular cell adhesion molecule-1 is expressed in human coronary atherosclerotic plaques: Implications for the mode of progression of advanced coronary atherosclerosis.J Clin Invest. 1993; 92: 945-951
- Destruction thresholds of echogenic liposomes with clinical diagnostic ultrasound.Ultrasound Med Biol. 2007; 33: 797-809
- Sustained activation of vascular cells and leukocytes in the rabbit aorta after balloon injury.Circulation. 1993; 88: 1788-1803
- Arterial thrombosis induces early upregulation of intercellular adhesion molecule in the media.J Vasc Surg. 1997; 26: 663-669
- Enhanced endothelial adhesiveness in hypercholesterolemia is attenuated by L-arginine.Circulation. 1994; 89: 2176-2182
- Adhesion molecules on the endothelium and mononuclear cells in human atherosclerotic lesions.Am J Pathol. 1992; 141: 1427-1433
Article info
Publication history
Published online: March 25, 2015
Accepted:
February 9,
2015
Received in revised form:
December 19,
2014
Received:
June 30,
2014
Identification
Copyright
© 2015 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.
