Abstract
Quantitative contrast-enhanced ultrasound plays an important role in tumor characterization
and treatment assessment. Besides established functional ultrasound techniques, ultrasound
molecular imaging using microbubbles targeted to disease-associated markers is increasingly
being applied in pre-clinical studies. Often, repeated injections of non-targeted
or targeted microbubbles during the same imaging session are administered. However,
the influence of repeated injections on the accuracy of the quantitative data is unclear.
Therefore, in tumor-bearing mice, we investigated the influence of multiple injections
of non-targeted microbubbles (SonoVue) on time to peak and peak enhancement in liver
and tumor tissue and of vascular endothelial growth factor receptor 2 (VEGFR2)-targeted
contrast agents (MicroMarker) on specific tumor accumulation. We found significantly
decreasing values for time to peak and a tendency for increased values for peak enhancement
after multiple injections. Repeated injections of VEGFR2-targeted microbubbles led
to significantly increased tumor accumulation, which may result from the exposure
of additional binding sites at endothelial surfaces caused by mechanical forces from
destroyed microbubbles.
Key Words
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Article info
Publication history
Published online: July 09, 2014
Accepted:
April 21,
2014
Received in revised form:
April 4,
2014
Received:
June 26,
2013
Identification
Copyright
© 2014 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.
