Abstract
Echocardiography is a reliable and commonly used method to examine cardiac diseases.
Recent employment of modern technologies provides new opportunities to study left
ventricular (LV) remodeling after myocardial infarction (MI) also in small rodents.
LV volumes as most important prognostic parameters can be estimated by noncontrast
enhanced echocardiography in rats from M-mode or single cross sections only. In this
study, contrast enhanced echocardiography and volume measurements by the biplane method
of discs (Simpson’s rule) were applied in rats to monitor remodeling and function
after MI. MI was induced in female Sprague-Dawley rats (n = 26 for MI, and n = 16 for sham). LV remodeling and heart function were serially studied by contrast
enhanced echocardiography for 12 to 16 wk. At the end of the observation periods hemodynamic
data were additionally measured by left and right heart catheterization. LV end systolic
volume (LVESV) measured by biplane method of discs correlated best with LV developed
pressure as indicator for severely impaired heart function. Interestingly, LV end
systolic area (LVESA) from native short axis view correlated well with LVESV (R2 = 0.93) and was the second best predictor for depressed heart function. Moreover,
left atrial size was a powerful indicator of severely impaired heart function whereas
ejection fraction or fractional area change were primarily related to infarct size.
In conclusion, contrast enhanced echocardiography in rats is feasible and an economical
method to study time-dependent LV remodeling and deterioration of contractile function
after MI. (E-mail: [email protected])
Key Words
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Article info
Publication history
Published online: July 09, 2007
Accepted:
April 26,
2007
Received in revised form:
April 22,
2007
Received:
October 25,
2006
Footnotes
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Identification
Copyright
© 2007 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.
