Abstract
Aneurysms remain a significant medical problem and our current understanding of aneurysm
formation and developmental stages remains incomplete. Noninvasive 3-D micro-ultrasound
(3-D micro-US) imaging technologies designed for noninvasive evaluation of small laboratory
animals diminish risks associated with invasive examination and provide in-situ (live) analysis of vascular morphological changes, which enables quantitative measurements
of live biological specimens. We demonstrate here that aneurysm morphology can be
quantified using 3-D micro-US, and we validate this methodology through comparison
of geometric measures with those obtained from 3-D serial histologic records in a
mouse model of accelerated aneurysm formation. Aneurysms were induced in Balb/C mice
after C57Bl/6 mouse aortic transplant with injections of a pro-inflammatory viral
serpin with a mutated reactive site. Aortic transplant segments were imaged 28 days
after transplant using 3-D micro-US. Upon sacrifice, the aortas were excised and histology
sections (5-μm thick) were digitized, co-registered using mutual information and stacked
to form 3-D images. Surfaces of the mouse aorta and aneurysm were manually segmented
from the 3-D micro-US and histology images. Comparisons with 3-D histology images
demonstrated that 3-D micro-US allowed in-vivo analysis of aneurysm morphology, including total aneurysm area, plaque growth and
lumen size. Linear regression of 3-D US-derived aneurysm and plaque volumes vs. 3-D histology-derived volumes resulted in slopes of 1.30 (R2 = 0.96) and 1.20 (R2 = 0.98), respectively, demonstrating that 3-D micro-US measurements can be used to
track aneurysm growth in a mouse aortic transplant model. (E-mail: [email protected])
Key Words
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References
- 3D ultrasound measurement of change in carotid plaque volume: A tool for rapid evaluation of new therapies.Stroke. 2005; 36: 1904-1909
- Elastin degradation and calcification in an abdominal aorta injury model: Role of matrix metalloproteinases. 2004; 110: 3480-3487
- Statistical methods for assessing agreement between two methods of clinical measurement.Lancet. 1986; 1: 307-310
- Three-dimensional ultrasound biomicroscopy for xenograft growth analysis.Ultrasound Med. Biol. 2005; 31: 865-870
- Serp-1, a viral anti-inflammatory serpin, regulates cellular serine proteinase and serpin responses to vascular injury.J Biol Chem. 2003; 278: 18563-18572
- Identification of myxomaviral serpin reactive site loop sequences that regulate innate immune responses.J Biol Chem. 2006; 281: 8041-8050
- Chronic angiotensin II infusion promotes atherogenesis in low density lipoprotein receptor −/− mice.Ann N Y Acad Sci. 1999; 892: 108-118
- Angiotensin II promotes atherosclerotic lesions and aneurysms in apolipoprotein E-deficient mice.J Clin Invest. 2000; 105: 1605-1612
- Urokinase-type plasminogen activator plays a critical role in angiotensin II-induced abdominal aortic aneurysm.Circ Res 92. 2003; 5: 510-517
- Three-dimensional US imaging.Phys Med Biol. 2001; 46: R67-R99
- High-frequency Doppler US monitors the effects of antivascular therapy on tumor blood flow.Cancer Res. 2002; 62: 6371-6375
- B-mode compound imaging in mice.Ultrasound Med Biol. 2006; 32: 29-32
- A swept-scanning mode for estimation of blood velocity in the microvasculature.IEEE Trans Ultrason Ferroelectr Freq Control. 1998; 45: 1437-1440
- Theoretical and experimental quantification of carotid plaque volume measurements made by three-dimensional ultrasound using test phantoms.Med Phys. 2002; 29: 2319-2327
- Measurement of carotid plaque volume by 3-dimensional ultrasound.Stroke. 2004; 35: 864
- Inflammation in atherosclerosis.Nature. 2002; 420: 868-874
- Noninvasive measurement of abdominal aortic aneurysms in intact mice by a high-frequency US imaging system.Ultrasound Med Biol. 2005; 31: 745-749
- Mutual-information-based registration of medical images: A survey.IEEE Trans Med Imaging. 2003; 22: 986-1004
- Quantitative evaluation of carotid plaque composition by in vivo MRI.Arterioscler Thromb Vasc Biol. 2005; 25: 234-239
- Inflammation and cellular immune responses in abdominal aortic aneurysms.Arterioscler Thromb Vasc Biol. 2006; 26: 987-994
- Aneurysm treatments expand.Nat Med. 2005; 11: 1279-1281
- US backscatter microscope analysis of mouse melanoma progression.Ultrasound Med Biol. 1996; 22: 845-853
- Angiotensin II increases urokinase-type plasminogen activator expression and induces aneurysm in the abdominal aorta of apolipoprotein E-deficient mice.Am J Pathol. 2001; 159: 1455-1464
- A new three-dimensional US micro-imaging technology for preclinical studies using a transgenic prostate cancer mouse model.Cancer Res. 2005; 65: 6337-6345
- Volume measurement variability in three-dimensional high-frequency ultrasound images of murine liver metastases.Phys Med Biol. 2006; 51: 2367-2381
Article info
Publication history
Published online: June 30, 2007
Accepted:
April 10,
2007
Received in revised form:
February 18,
2007
Received:
September 9,
2006
Identification
Copyright
© 2007 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.