Abstract
Despite considerable knowledge about effects of extracorporeal shock-wave therapy
(ESWT) on eukaryotic tissues, only little data are available concerning their effect
on prokaryotic microorganisms. The objective of the present study was to determine
the bactericidal activity as a function of energy flux density and shock-wave impulse
number. Standardised suspensions of Staphylococcus aureus ATCC 25923 were exposed to different impulse numbers of shock waves with an energy
flux density (ED) up to 0.96 mJ mm−2 (2 Hz). Subsequently, viable bacteria were quantified by culture and compared with
an untreated control. After applying 4000 impulses, a significant bactericidal effect
was observed with a threshold ED of 0.59 mJ mm−2 (p < 0 · 05). A threshold impulse number of more than 1000 impulses was necessary to
reduce bacterial growth (p < 0 · 05). Further elevation of energy and impulse number exponentially increased
bacterial killing. ESWT proved to exert significant antibacterial effect in an energy-dependent
manner. Certain types of difficult-to-treat infections could offer new applications
for ESWT. (E-mail: [email protected])
Key words
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Article info
Publication history
Accepted:
August 26,
2004
Received in revised form:
August 23,
2004
Received:
May 10,
2004
Identification
Copyright
© 2005 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.
