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Abstract
The induction of DNA strand breaks in Chinese hamster ovary cells was measured with
the comet assay after continuous wave ultrasound or lithotripter shock wave exposure.
Cell lysis and hydrogen peroxide production were measured to gauge the level of inertial
cavitation activity. Significant DNA damage was found after 2.17-MHz ultrasound exposure
at 37°C to 0.82 MPa for 2 min or 4 min, and to 0.58 MPa for 4 min. A significant portion
of the damage induced at the 0.82-MPa level was repaired by the cells when warmed.
Neither exposure to 500 or 1000 shock waves at 37°C in a thin-walled tube, nor exposure
to 1000, 1500 or 2000 shock waves at 25°C in a polyethylene pipette bulb produced
a significant effect, when the flash of light from the spark-gap discharge was blocked.
This finding was consistent with the generally lower lysis and hydrogen peroxide production
by the shock wave exposure.
Keywords
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References
- Effects of piezoelectric lithotripsy on human DNA.Ultrasound Med Biol. 1995; 21: 399-403
- Effect of shock waves on suspended and immobilized L1210 cells.Ultrasound Med Biol. 1989; 15: 229-239
- A Review of the physical properties and biological effects of the high amplitude acoustic fields used in extracorporeal lithotripsy.Ultrasonics. 1993; 31: 75-89
- Medical applications and bioeffects of extracorporeal shock waves.Shock Waves. 1994; 4: 55-72
- Flow cytometric analysis of cell suspensions exposed to shock waves in the presence of the radical sensitive dye hydroethidine.Ultrasound Med Biol. 1995; 21: 569-577
- Influence of dissolved and free gases on iodine release and cell killing by shock waves in vitro.Ultrasound Med Biol. 1992; 18: 617-623
- Biological effects of shock waves: Cell disruption, viability, and proliferation of L1210 cells exposed to shock waves in vitro.Ultrasound Med Biol. 1990; 16: 589-594
- DNA damaging activity of cadmium in Leydig cells, a target cell population for cadmium carcinogenesis in the rat testis.Toxicology Letters. 1992; 63: 211-220
- Free radical formation induced by ultrasound and its effects on strand breaks in DNA of cultured FM3A cells.Free Rad Res Comms. 1993; 19 (Suppl): S193-S200
- Apoptosis of murine BW 5147 thymoma cells induced by cold shock.J Cell Physiol. 1992; 153: 112-117
- A comparison of hemolytic and sonochemical activity of ultrasonic cavitation in a rotating tube.Ultrasound Med Biol. 1993; 19: 83-90
- Thresholds for hemorrhages in mouse skin and intestine induced by lithotripter shock waves.Ultrasound Med Biol. 1995; 21: 249-257
- Comet assay reveals DNA strand breaks induced by ultrasonic cavitation in vitro.Ultrasound Med Biol. 1995; 21: 841-848
- Ultrasonic cavitation indirectly induces single strand breaks in DNA of viable cells in vitro by the action of residual hydrogen peroxide.Ultrasound Med Biol. 1991; 17: 729-735
- Single strand breaks in CHO cell DNA induced by ultrasonic cavitation in vitro.Ultrasound Med Biol. 1991; 17: 401-406
Miller DL, Thomas RM, Thrall BD. The role of ultraviolet light in the induction of cellular DNA damage by a spark-gap lithotripter in vitro. J Urology (In press)
- Nucleation and evolution of ultrasonic cavitation in a rotating exposure chamber.J Ultras Med. 1992; 11: 407-412
Miller MW, Miller DL, Brayman AA. A mechanistic analysis of bioeffects of inertial cavitation in vitro. (In press)
- Free radical production by high energy shock waves-comparison with ionizing irradiation.J Urology. 1988; 139: 186-189
- Cytotoxic effects of acoustic cavitation on HT-29 cells and a rat peritoneal carcinomatosis in vitro.Cancer Res. 1991; 51: 3024-3029
- The in vitro and in vivo effects of extracorporeal shock waves on malignant cells.Urological Res. 1988; 16: 419-426
- Free radical formation induced by ultrasound and its biological implications.Free Radical Biology & Medicine. 1992; 13: 247-270
- Induction and rejoining of DNA single-strand breaks in relation to cellular growth in human cells exposed to three hydroperoxides at 0°C and 37°C.Free Rad Res Comms. 1991; 15: 79-89
- A simple technique for quantitation of low levels of dna damage in individual cells.Experimental Cell Res. 1988; 175: 184-191
- Cytotoxic effects of high energy shock waves in different in vitro models: Influence of the experimental set-up.J Urology. 1991; 145: 171-175
- In vitro investigations on cellular damage induced by high energy shockwaves.Ultrasound Med Biol. 1992; 18: 691-699
- Cavitation-generated free radicals during shock wave exposure: Investigations with cell-free solutions and suspended cells.Ultrasound Med Biol. 1991; 17: 761-768
- Reduced cavitation-induced cellular damage by the antioxidative effect of vitamin E.Ultrasonics. 1994; 32: 301-308
- Histidine modulates the clastogenic effect of oxidative stress.Mutation Research. 1989; 211: 103-109
- Assessment of radiation-induced DNA damage in human blood lymphocytes using the single cell gel electrophoresis technique.Mutation Res. 1992; 271: 243-252
- A test of the hypothesis that cavitation at the focal area of an extracorporeal shock wave lithotripter produces far ultraviolet and soft x-ray emissions.J Acoust Soc Am. 1995; 98: 706-711
Article info
Publication history
Accepted:
November 15,
1995
Received:
August 21,
1995
Identification
Copyright
© 1996 Published by Elsevier Inc.
