Ultrasound in Medicine and Biology
Volume 36, Issue 3 , Pages 449-458 , March 2010

Ultrasonic Microbubble-Mediated Gene Delivery Causes Phenotypic Changes of Human Aortic Endothelial Cells

  • Cheng-Huang Su

      Affiliations

    • Departments of Internal Medicine and Medical Research, Mackay Memorial Hospital, Taipei City, Taiwan
    • Mackay Medicine, Nursing and Management College/Mackay Medical College, Taipei City, Taiwan
    • ,
  • Chiung-Yin Chang

      Affiliations

    • Departments of Internal Medicine and Medical Research, Mackay Memorial Hospital, Taipei City, Taiwan
    • ,
  • Hsueh-Hsiao Wang

      Affiliations

    • Departments of Internal Medicine and Medical Research, Mackay Memorial Hospital, Taipei City, Taiwan
    • ,
  • Yih-Jer Wu

      Affiliations

    • Departments of Internal Medicine and Medical Research, Mackay Memorial Hospital, Taipei City, Taiwan
    • ,
  • Thierry Bettinger

      Affiliations

    • Novel Agents Research Dept, Bracco Research Geneva, Plan-les-Ouates, Geneva, Switzerland
    • ,
  • Cheng-Ho Tsai

      Affiliations

    • Departments of Internal Medicine and Medical Research, Mackay Memorial Hospital, Taipei City, Taiwan
    • ,
  • Hung-I Yeh

      Affiliations

    • Departments of Internal Medicine and Medical Research, Mackay Memorial Hospital, Taipei City, Taiwan
    • Corresponding Author InformationAddress correspondence to: Hung-I Yeh, MD, PhD, FESC, Departments of Internal Medicine and Medical Research, Mackay Memorial Hospital, Mackay Medical College, No. 46, Sec. 3, Jhong-Jheng Road, San-Jhih Township, Taipei County 252, Taiwan.

Received 23 June 2009 ,Revised 13 November 2009 ,Accepted 20 November 2009.

References 

  1. Abdollahi A, Domhan S, Jenne JW, Hallaj M, Dell'Aqua G, Mueckenthaler M, et al. Apoptosis signals in lymphoblasts induced by focused ultrasound. FASEB J. 2004;18:1413–1414
  2. Feril LB, Kondo T, Zhao QL, Ogawa R, Tachibana K, Kudo N, et al. Enhancement of ultrasound-induced apoptosis and cell lysis by echo-contrast agents. Ultrasound Med Biol. 2003;29:331–337
  3. Gonzalez MA, Selwyn AP. Endothelial function, inflammation, and prognosis in cardiovascular disease. Am J Med. 2003;115(Suppl. 8A):99S–106S
  4. Grazia Lampugnani M, Zanetti A, Corada M, Takahashi T, Balconi G, Breviario F, et al. Contact inhibition of VEGF-induced proliferation requires vascular endothelial cadherin, beta-catenin, and the phosphatase DEP-1/CD148. J Cell Biol. 2003;161:793–804
  5. Hadi HA, Carr CS, Al Suwaidi J. Endothelial dysfunction: cardiovascular risk factors, therapy, and outcome. Vasc Health Risk Manag. 2005;1:183–198
  6. Hallow DM, Mahajan AD, Prausnitz MR. Ultrasonically targeted delivery into endothelial and smooth muscle cells in ex vivo arteries. J Control Release. 2007;118:285–293
  7. Landmesser U, Drexler H. The clinical significance of endothelial dysfunction. Curr Opin Cardiol. 2005;20:547–551
  8. Lawrie A, Brisken AF, Francis SE, Tayler DI, Chamberlain J, Crossman DC, et al. Ultrasound enhances reporter gene expression after transfection of vascular cells in vitro. Circulation. 1999;99:2617–2620
  9. Lohse D. Sonoluminescence: Cavitation hots up. Nature. 2005;434:33–34
  10. Lu J, Lu Z, Reinach P, Zhang J, Dai W, Lu L, et al. TGF-beta2 inhibits AKT activation and FGF-2-induced corneal endothelial cell proliferation. Exp Cell Res. 2006;312:3631–3640
  11. McNeil PL. Incorporation of macromolecules into living cells. Methods Cell Biol. 1989;29:153–173
  12. Meijering BD, Henning RH, Van Gilst WH, Gavrilovic I, Van Wamel A, Deelman LE. Optimization of ultrasound and microbubbles targeted gene delivery to cultured primary endothelial cells. J Drug Target. 2007;15:664–671
  13. Mizrahi N, Seliktar D, Kimmel E. Ultrasound-induced angiogenic response in endothelial cells. Ultrasound Med Biol. 2007;33:1818–1829
  14. Newman CM, Bettinger T. Gene therapy progress and prospects: ultrasound for gene transfer. Gene Ther. 2007;14:465–475
  15. Raz D, Zaretsky U, Einav S, Elad D. Cellular alterations in cultured endothelial cells exposed to therapeutic ultrasound irradiation. Endothelium. 2005;12:201–213
  16. Sakurai Y, Ohgimoto K, Kataoka Y, Yoshida N, Shibuya M. Essential role of Flk-1 (VEGF receptor 2) tyrosine residue 1173 in vasculogenesis in mice. Proc Natl Acad Sci U S A. 2005;102:1076–1081
  17. Tachibana K, Uchida T, Ogawa K, Yamashita N, Tamura K. Induction of cell-membrane porosity by ultrasound. Lancet. 1999;353:1409
  18. Taniyama Y, Tachibana K, Hiraoka K, Aoki M, Yamamoto S, Matsumoto K, et al. Development of safe and efficient novel nonviral gene transfer using ultrasound: Enhancement of transfection efficiency of naked plasmid DNA in skeletal muscle. Gene Ther. 2002;9:372–380
  19. Taniyama Y, Tachibana K, Hiraoka K, Namba T, Yamasaki K, Hashiya N, et al. Local delivery of plasmid DNA into rat carotid artery using ultrasound. Circulation. 2002;105:1233–1239
  20. Tomiya T, Ogata I, Yamaoka M, Yanase M, Inoue Y, Fujiwara K. The mitogenic activity of hepatocyte growth factor on rat hepatocytes is dependent upon endogenous transforming growth factor-alpha. Am J Pathol. 2000;157:1693–1701
  21. Vannan M, McCreery T, Li P, Han Z, Unger E, Kuersten B, et al. Ultrasound-mediated transfection of canine myocardium by intravenous administration of cationic microbubble-linked plasmid DNA. J Am Soc Echocardiogr. 2002;15:214–218
  22. Vinals F, Pouyssegur J. Confluence of vascular endothelial cells induces cell cycle exit by inhibiting p42/p44 mitogen-activated protein kinase activity. Mol Cell Biol. 1999;19:2763–2772
  23. Wang HH, Kung CI, Tseng YY, Lin YC, Chen CH, Tsai CH, et al. Activation of endothelial cells to pathological status by down-regulation of connexin43. Cardiovasc Res. 2008;79:509–518
  24. Wang Y, Xu HX, Lu MD, Tang Q. Expression of thymidine kinase mediated by a novel non-viral delivery system under the control of vascular endothelial growth factor receptor 2 promoter selectively kills human umbilical vein endothelial cells. World J Gastroenterol. 2008;14:224–230
  25. Zachary I. Neuroprotective role of vascular endothelial growth factor: signalling mechanisms, biological function, and therapeutic potential. Neurosignals. 2005;14:207–221
  26. Zou H, Nie XH, Zhang Y, Hu M, Zhang YA. Effect of basic fibroblast growth factor on the proliferation, migration and phenotypic modulation of airway smooth muscle cells. Chin Med J (Engl). 2008;121:424–429

PII: S0301-5629(09)01654-8

doi: 10.1016/j.ultrasmedbio.2009.11.006

Ultrasound in Medicine and Biology
Volume 36, Issue 3 , Pages 449-458 , March 2010

Article Tools

* Image Usage & Resolution