Ultrasound in Medicine and Biology
Volume 35, Issue 10 , Pages 1638-1646 , October 2009

Partial Restoration of Left Ventricular Systolic Function by asPLB Gene Transfer Using Ultrasound-Mediated Microbubble Destruction

  • Yun Mou

      Affiliations

    • Institute of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, P.R. China
    • ,
  • Yang Ye

      Affiliations

    • Institute of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, P.R. China
    • ,
  • Xiao-Yan Zhao

      Affiliations

    • Institute of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, P.R. China
    • ,
  • Lei Yao

      Affiliations

    • Institute of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, P.R. China
    • ,
  • Li-Ping Yan

      Affiliations

    • Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, P.R. China
    • ,
  • Jian Sun

      Affiliations

    • Institute of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, P.R. China
    • ,
  • Zhao-Hui Zhu

      Affiliations

    • Institute of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, P.R. China
    • ,
  • Shen-Jiang Hu

      Affiliations

    • Institute of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, P.R. China
    • Division of Nitric Oxide and Inflammatory Medicine, E-Institute of Shanghai Universities, Shanghai, P.R. China
    • Corresponding Author InformationAddress correspondence to: Shen-Jiang Hu, Institute of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, #79 Qingchun Road, Hangzhou 310003, P.R. China.

Received 18 June 2008 ,Revised 21 March 2009 ,Accepted 8 April 2009.

References 

  1. Bekeredjian R, Chen S, Frenkel PA, Grayburn PA, Shohet RV. Ultrasound-targeted microbubble destruction can repeatedly direct highly specific plasmid expression to the heart. Circulation. 2003;108:1022–1026
  2. Bekeredjian R, Grayburn PA, Shohet RV. Use of ultrasound contrast agents for gene or drug delivery in cardiovascular medicine. J Am Coll Cardiol. 2005;45:329–335
  3. Champion HC, Georgakopoulos D, Haldar S, Wang L, Wang Y, Kass DA. Robust adenoviral and adeno-associated viral gene transfer to the in vivo murine heart: Application to study of phospholamban physiology. Circulation. 2003;108:2790–2797
  4. Chen S, Shohet RV, Bekeredjian R, Frenkel P, Grayburn PA. Optimization of ultrasound parameters for cardiac gene delivery of adenoviral or plasmid deoxyribonucleic acid by ultrasound-targeted microbubble destruction. J Am Coll Cardiol. 2003;42:301–308
  5. Chu G, Kranias EG. Functional interplay between dual site phospholamban phosphorylation: Insights from genetically altered mouse models. Basic Res Cardiol. 2002;97(Suppl 1):I43–I48
  6. del Monte F, Harding SE, Dec GW, Gwathmey JK, Hajjar RJ. Targeting phospholamban by gene transfer in human heart failure. Circulation. 2002;105:904–907
  7. Ding Z, Fach C, Sasse A, Gödecke A, Schrader J. A minimally invasive approach for efficient gene delivery to rodent hearts. Gene Ther. 2004;11:260–265
  8. Duffield JS, Bonventre JV. Kidney tubular epithelium is restored without replacement with bone marrow-derived cells during repair after ischemic injury. Kidney Int. 2005;68:1956–1961
  9. Eizema K, Fechner H, Bezstarosti K, Schneider-Rasp S, van der Laarse A, Wang H, et al. Adenovirus-based phospholamban antisense expression as a novel approach to improve cardiac contractile dysfunction: Comparison of a constitutive viral versus an endothelin-1-responsive cardiac promoter. Circulation. 2000;101:2193–2199
  10. Iwanaga Y, Hoshijima M, Gu Y, Iwatate M, Dieterle T, Ikeda Y, et al. Chronic phospholamban inhibition prevents progressive cardiac dysfunction and pathological remodeling after infarction in rats. J Clin Invest. 2004;113:727–736
  11. Kadambi VJ, Ponniah S, Harrer JM, Hoit BD, Dorn GW, Walsh RA, et al. ardiac-specific overexpression of phospholamban alters calcium kinetics and resultant cardiomyocytes mechanics in transgenic mice. J Clin Invest. 1996;97:53–59
  12. Kondo I, Ohmori K, Oshita A, Takeuchi H, Fuke S, Shinomiya K, et al. Treatment of acute myocardial infarction by hepatocyte growth factor gene transfer: The first demonstration of myocardial transfer of a “functional” gene using ultrasonic microbubble destruction. J Am Coll Cardiol. 2004;44:644–653
  13. Larsen JS, Kjeldsen K. Quantification in crude homogenates of rat myocardial Na+, K+- and Ca2+-ATPase by K+ and Ca2+-dependent pNPPase. Age-dependent changes. Basic Res Cardiol. 1995;90:323–331
  14. Lawrie A, Brisken AF, Francis SE, Tayler DI, Chamberlain J, Crossman DC, et al. Ultrasound enhances reporter gene expression after transfection of vascular cells in vitro. Circulation. 1999;20:2617–2620
  15. Li J, Hu SJ, Sun J, Zhu ZH, Zheng X, Wang GZ, et al. Construction of phospholamban antisense RNA recombinant adenoassociated virus vector and its effects in rat cardiomyocytes. Acta Pham Sinica. 2005;26:51–55
  16. Li TS, Hayashi M, Ito H, Furutani A, Murata T, Matsuzaki M, et al. Regeneration of infarcted myocardium by intramyocardial implantation of ex vivo transforming growth factor-beta-preprogrammed bone marrow stem cells. Circulation. 2005;111:2438–2445
  17. Luo W, Grupp IL, Harrer J, Ponniah S, Grupp G, Duffy JJ, et al. Targeted ablation of the phospholamban gene is associated with markedly enhanced myocardial contractility and loss of β-agonist stimulation. Circ Res. 1994;75:401–409
  18. McTiernan CF, Frye CS, Lemster BH, Kinder EA, Ogletree-Hughes ML, Moravec CS, et al. The human phospholamban gene: Structure and expression. J Mol Cell Cardiol. 1999;31:679–692
  19. Matsumoto R, Omura T, Yoshiyama M, Hayashi T, Inamoto S, Koh KR, et al. Vascular endothelial growth factor-expressing mesenchymal stem cell transplantation for the treatment of acute myocardial infarction. Arterioscler Thromb Vasc Biol. 2005;25:1168–1173
  20. Miller DL, Quddus J. Diagnostic ultrasound activation of contrast agent gas bodies nduces capillary rupture in mice. Proc Natl Acad Sci USA. 2000;97:10179–10184
  21. Miller DL, Pislaru SV, Greenleaf JE. Sonoporation: Mechanical DNA delivery by ultrasonic cavitation. Somat Cell Mol Genet. 2002;27:115–134
  22. Pachori AS, Melo LG, Zhang L, Loda M, Pratt RE, Dzau VJ. Potential for germ line transmission after intramyocardial gene delivery by adeno-associated virus. Biochem Biophys Res Commun. 2004;313:528–533
  23. Periasamy M, Bhupathy P, Babu GJ. Regulation of sarcoplasmic reticulum Ca2+ATPase pump expression and its relevance to cardiac muscle physiology and pathology. Cardiovasc Res. 2008;15(77):265–273
  24. Roufosse C, Bou-Gharios G, Prodromidi E, Alexakis C, Jeffery R, Khan S, et al. Bone marrow-derived cells do not contribute significantly to collagen I synthesis in a murine model of renal fibrosis. J Am Soc Nephrol. 2006;17:775–782
  25. Sato M, O'Grara P, Harding SE, Fuller SJ. Enhancement of adenoviral gene transfer to adult rat cardiomyocytes in vivo by immobilization and ultrasound treatment of the heart. Gene Ther. 2005;12:936–941
  26. Sande JB, Sjaastad I, Hoen IB, Bøkenes J, Tønnessen T, Holt E, et al. Reduced level of serine16 phosphorylated phospholamban in the failing rat myocardium: A major contributor to reduced SERCA2 activity. Cardiovasc Res. 2002;53:382–391
  27. Schiller NB, Shah PM, Crawford M, DeMaria A, Devereux R, Feigenbaum H, et al. American Society of Echocardiography Committee on Standards, Subcommittee on Quantitation of Two-Dimensional Echocardiograms. Recommendations for quantitation of the left ventricle by two-dimensional echocardiography. J Am Soc Echocardiogr. 1989;2:358–367
  28. Schwinger RH, Münch G, Bölck B, Karczewski P, Krause EG, Erdmann E. Reduced Ca2+-sensitivity of SERCA 2a in failing human myocardium due to reduced serine-16 phospholamban phosphorylation. J Mol Cell Cardiol. 1999;31:479–491
  29. Sheehan FH, Bolson EL, Dodge HT, Mathey DG, Schofer J, Woo HW. Advantages and applications of the centerline method for characterizing regional ventricular function. Circulation. 1986;74:293–305
  30. Shohet RV, Chen S, Zhou YT, Wang Z, Meidell RS, Unger RH, et al. Echocardiographic destruction of albumin microbubbles directs gene delivery to the myocardium. Circulation. 2000;101:2554–2556
  31. Sjaastad I, Wasserstrom JA, Sejersted OM. Heart failure—A challenge to our current concepts of excitation-contraction coupling. J Physiol. 2003;546:33–47
  32. Sun YL, Hu SJ, Wang LH, Hu Y, Zhou JY. Comparison of low and high doses of carvedilol on restoration of cardiac function and calcium-handling proteins in rat failing heart. Clin Exp Pharmacol Physiol. 2005;32:553–560
  33. Taniyama Y, Tachibana K, Hiraoka K, Namba T, Yamasaki K, Hashiva N, et al. Local delivery of plasmid DNA into rat carotid artery using ultrasound. Circulation. 2002;105:1233–1239
  34. Traaseth NJ, Ha KN, Verardi R, Shi L, Buffy JJ, Masterson LR, et al. Structural and dynamic basis of phospholamban and sarcolipin inhibition of Ca(2+)-ATPase. Biochemistry. 2008;47:3–13
  35. Tsunoda S, Mazda O, Oda Y, Iida Y, Akabame S, Kishida T, et al. Sonoporation using microbubble BR14 promotes pDNA/siRNA transduction to murine heart. Biochem Biophys Res Commun. 2005;336:118–127
  36. Zhai J, Schmidt AG, Hoit BD, Kimura Y, MacLennan DH, Kranias EG. Cardiac-specific overexpression of a superinhibitory pentameric phospholamban mutant enhances inhibition of cardiac function in vivo. J Biol Chem. 2000;275:10538–10544
  37. Zhao XY, Hu SJ, Li J, Mou Y, Bian K, Sun J, et al. rAAV-asPLB transfer attenuates abnormal sarcoplasmic reticulum Ca(2+)-ATPase activity and cardiac dysfunction in rats with myocardial infarction. Eur J Heart Fail. 2008;10:47–54

PII: S0301-5629(09)00209-9

doi: 10.1016/j.ultrasmedbio.2009.04.018

Ultrasound in Medicine and Biology
Volume 35, Issue 10 , Pages 1638-1646 , October 2009

Article Tools

* Image Usage & Resolution