Ultrasound in Medicine and Biology
Volume 29, Issue 1 , Pages 93-102, January 2003

Biological and environmental factors affecting ultrasound-induced hemolysis in vitro: 2. medium dissolved gas (pO2) content

  • Morton W Miller

      Affiliations

    • Department of Obstetrics and Gynecology, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA
    • Corresponding Author InformationAddress correspondence to: Dr. M. W. Miller, Department of Obstetrics and Gynecology, School of Medicine & Dentistry, University of Rochester, Box 668, Rochester NY 14642-0001, USA.
    • ,
  • E.Carr Everbach

      Affiliations

    • Department of Engineering, Swarthmore College, Swarthmore, PA, USA
    • ,
  • W.Marcus Miller

      Affiliations

    • Varian NMR Systems, Palo Alto, CA, USA
    • ,
  • Linda F Battaglia

      Affiliations

    • Department of Obstetrics and Gynecology, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA

Received 25 February 2002; accepted 27 May 2002.

Abstract 

The data collected in this project supported the a priori hypothesis that the concentration of dissolved oxygen in whole human blood in vitro affected the extent of ultrasound (US)-induced hemolysis under conditions conducive to the occurrence of inertial cavitation. Aliquots of whole human blood in vitro with a relatively high O2 level had statistically significantly more 1-MHz US-induced hemolysis than aliquots with a relatively low O2 level in the presence of controlled gas nucleation (Albunex® or ALX, supplementation), with US-induced hemolytic yields being substantially less at 2.2- and 3.5-MHz exposures or in the absence of ALX-supplementation at otherwise comparable acoustic pressures, pulse lengths and duty factors. Passive cavitation detection (pcd) measures indicated a linear relationship for hemolysis up to about 70% and pcd values (R2 = 0.99). (E-mail: Morton_Miller@urmc.rochester.edu)

Keywords:  Hemolysis, In vitro, Partial pressure of oxygen, Inertial cavitation

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0301-5629(02)00562-8

doi:10.1016/S0301-5629(02)00562-8

Ultrasound in Medicine and Biology
Volume 29, Issue 1 , Pages 93-102, January 2003

Article Tools

* Image Usage & Resolution